Biochemical Aspects of Toxicological Studies of Azadirachta Indica (A.Juss).

Anyabuike, Chinedum Peace (1986)

Thesis

Azedirachta indica A. Juss, family, Meliaceae, is a medicinal plant used by some people for the treatment of malaria and inflammatory diseases in Africa and Asia. Intensive research into the application of this plant as an anti-feedant in agriculture is currently being carried out in some parts of the world. It has been shown in this work that the aqueous extract of the leaves caused acute hepatobiliary disease condition as revealed by monitoring 5 nucleotides activity in the serum of male rat. The toxic effect was found to be dose-dependent. This toxic effect was lost with time as a result of a probable regeneration of the liver 2-le effect of pretreatment of the rats with classical mixed function oxidases inducers and inhibitors on the hepatotoxicity of the Azadirachta indica leaf extract was carrried out. For the inducers, phenobarbitone sodium neither reduced nor enhanced the hepatotoxicit alcohol and anthracene reduced the hepatotoxicity. For the inhibitors, cimetidine and metyrapone greatly reduced the hepatotoxicity. Mixed function oxidases (MMFO) have been implicated in hepatotoxicity due to of aflatoxin B1, carbon tetrachloride, bromobenzene and thioacetamide where anthracene which is an enzyme inducer depressed the hepatotoxicity and known inhibitors like metyrapone and SKF 52S-A greatly depressed the hepatotoxicity. It may therefore be concluded from the above information that mixed function oxidases may play a role in the hepatotoxicity of aqueous extract of the leaves of Azadirachta indica A. Juss.

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