Pharmacological Analysis of the Neuromuscular Properties of Diethylcarbamazine Citrate in Vitro.

Olayiwola, Gbola (1985)

Thesis

The pharmacological actions of diethylcarbamazine citrate (DECC) have been examined in vitro on isolated chick biventer-cervicis muscle preparations. DECC (5x10-1 - 1.5mM) produced a concentration-related initial augmentation of the indirect electrically-evoked twitches followed by a sustained, longer-lasting secondary depression of the twitches of the skeletal muscle-nerve (chick biventer-cervicis) preparations examined. DECC-induced twitch depression was competitively reversed by increasing calcium ion (Ca ) concentration of the bathing fluid. The contractual responses of innervated chick biventor m muscle preparation induced by bath-applied low concentrations (2.5x10-2M) of carbachol, acetylcholine or nicotine was markedly augmented by DECC (10-3 - 2.5x10-2M). This same concentration of DECC (10-3 - 2.5x10-2M)• also depressed the contra contractual responses of the muscle elicited by high concentrations of the agonists. Relatively high concentrations of DECC (10-1 - 2.5mM) dose dependently contracted isolated chick biventer cervicis muscle preparations, and demonstrated measurable anticholinesterase activity. It is concluded that DECC possesses both direct (post-junctional) and indirect (pre-junctional) effects at the neuromuscular junction, and that the neuromuscular blockade produced by DECC is possibly post-junctional in origin. The clinical significance of the neuromuscular blockade produced by DECC is also discussed.

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