Master of Science (M.Sc.) Theses and Dissertations

Permanent URI for this collection

https://ir.oauife.edu.ng/handle/123456789/6251

Browse

Browsing Master of Science (M.Sc.) Theses and Dissertations by Author "Babarinde, Ganiyat Adeola."

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Open Access
    The Phytochemical screening , Antimicrobial analysis and Mechanism of action Acalypha Wilkesiana and Senna Alata Leaf extracts on selected Pathogenic Microorganisms.
    (Department of Microbiology. Faculty of Science, 2023) Babarinde, Ganiyat Adeola.
    This study extracted and identified the phytochemical constituents of the leaves of Acalypha wilkesiana and Senna alata, assessed the antimicrobial effect of the leaf extracts on selected pathogenic microorganisms and determined the mechanism of action of the extracts on selected the pathogenic microorganisms. These were with a view to providing information on the phytochemical constituents and antimicrobial activity of Acalypha wilkesiana and Senna alata leaf extracts against selected pathogenic microorganisms. Fresh young leaf petals of Senna alata and Acalypha wilkesiana were collected, washed, and dried in hot-air oven at 40oC to obtain a constant weight. The plant sample were homogenized into fine powder. The dried powder was extracted for 72 hours using methanol and distilled water respectively. The extract was then fractionized using n-hexane, butanol, ethyl acetate and water. The solvent was eliminated using evaporator and lyophilized. These fractions were analysed for their bioactive compounds. Antimicrobial analysis was carried out on the extracts with some selected clinical isolates of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 87853, Klebsiella pneumoniae, Klebsiella oxytoca, Acinetobacter baumannii, Aspergillus brevipes and Candida albican. from different infectious sources for their antimicrobial activities. Antimicrobial activity of the plant extracts was analysed to determine the zone of inhibition as well as the minimum inhibitory concentration and minimum bactericidal/ fungicidal concentration. These extracts were further analysed for their mechanism of action with some standard antibiotics. The mechanism of action of the fractionated extracts of the plants were tested in combination with standard antibiotics (Streptomycin. Ampicillin and Fluconazole).