Browsing by Author "Arowosegbe, Sunday Micheal"

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    Isolation, characterization and bioactivity studies of the extract from aerial part of hilleria latifolia (LAM)
    (Department of Chemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife., 2023) Arowosegbe, Sunday Micheal
    This study screened the crude extract of Hilleria latifolia for its phytochemical constituents and evaluated the extract and fractions for their antioxidant and antibacterial activities. It also isolated compound from its active fraction and elucidated the structure of the compound. The antioxidant and antibacterial potential of the isolated compound were also determined. These were with a view to finding novel bioactive compound from this medicinal plant. The aerial parts of the plant was air-dried and pulverized. The pulverized plant was extracted with 90% methanol/water for 96 hours and filtered. The filtrate obtained was concentrated to dryness in vacuo at 40 ℃. This afforded the crude extract which was subjected to solvent partitioning using solvents of varying polarities: n-hexane, dichloromethane and ethyl acetate in turn. This yielded four solvent fractions, and the aqueous MeOH fraction inclusive. The crude and the solvent fractions were tested for antibacterial activity, Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and antioxidant activities using a number of antioxidant assays namely: 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Nitric Oxide (NO) Scavenging, Metal Chelating Ability (MCA), Ferric Reducing Antioxidant Power (FRAP) and Total Antioxidant Capacity (TAC). The antibacterial activity was evaluated using the Agar diffusion method. The results showed that of all the solvent fractions obtained, the ethyl acetate fraction was the most active in the DPPH, TAC, MCA, and FRAP assays. One compound was isolated from the aqueous fraction using column chromatography. The isolated compound, HLA-1 was characterized by Nuclear Magnetic Resonance (NMR) and Infrared (IR) spectroscopy. Compound HLA-1 was identified as 8 – methyl – 1, 4 – oxazo – 2 – enine – 5, 7, 9 – trione. The n–hexane and dichloromethane fraction obtained from the crude extracts of Hilleria latifolia exhibited significant biological and antioxidant activities. These fractions were subjected to GC – MS analyses and a total of 27 compounds were identified in both fractions. Most of the identified constituents have been reported to possess various biological activities such as antioxidant, anticancer, anti-inflammatory and antibacterial activities. The study concluded that the extracts and isolated compound from Hilleria latifolia exhibited varying degree of antibacterial and antioxidant activities and could be effective in the management of infectious and oxidative stress related diseases. These observed activities could justify the ethnomedicinal uses of the plant.