Browsing by Author "GBOTOSHO Olabisi Abosede"

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    Evaluation of serum free light chains as markers of disease severity in patients with chronic lymphocytic leukamia in southwestern Nigeria.
    (Department of Haematology and Immunlogy, Faculty of Basic Medical Science, Obafemi Awolowo University., 2022) GBOTOSHO Olabisi Abosede
    The aim of this study is to determine the haematological parameters, disease severity, serum lactate dehydrogenase, serum β2 microglobulin, serum creatinine and serum free light chains kappa and lambda of newly diagnosed CLL patients; and assess the relationship between the pattern of free light chains and the measured parameters, with the view to improve treatment of chronic lymphocytic leukemia patients. Thirty-three newly diagnosed CLL patients were enrolled into the study using convenience sampling method. Patients with other chronic illnesses or in disease transformation were excluded. Ethical approval was obtained from the National Health Research Ethics Committee of Nigeria. Data on socio-demographic characteristics, general physical and systemic examination was also collected for each eligible patient, after informed consent was obtained. Clinical disease severity score was determined using the Binet staging system. Ten milliliters of venous blood were collected; 5ml were anticoagulated using EDTA to obtain haematological parameters from an haemo-autoanalyzer; and for making peripheral blood film using standard operating procedures. The remaining 5ml of blood was dispensed into a plain bottle to obtain serum using standard operating procedures. The sera collected were stored at -200C and batch tested for serum free light chains, kappa and lambda, from which the kappa/lambda ratio was calculated, β2-microglobulin, lactate dehydrogenase (LDH) and creatinine. Numerical values were reported as frequency, percentage, median, interquartile range, minimum and maximum values. Bivariate analysis was used for continuous variables while chi-square test was used for non-continuous variables data. A p-value ≤ 0.05 or 95% CI (CI=95%) was defined as statistical significance