Browsing by Author "Omoboye, Olumide"

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    Open Access
    Effects of different substrates on the morphological characteristics and proximate content of Pleurotus pulminarius. Food Science and Quality Management
    (international journal of proximate content of Pleurotus pulminarius, 2014-10) Bakare, Mufutau Kolawole; A. O., Oluduro; Omoboye, Olumide; Leramo, Adefemi Joseph
    Morphological characteristics and proximate content of Pleurotus pulminarius cultivated on different substrates were investigated. The substrates used are cotton waste, sawdust, palm fruit waste, paper, mixture of sawdust and palm fruit waste and mixture of cotton waste and paper (1:1) were sterilized and inoculated with Pleurotus pulminarius spawn (sorghum-produced) aseptically, incubated under controlled temperature and humidity before exposure to sunlight. Length and, diameter of stalk and pileus were measured during stages of harvest.Protein, fat, carbohydrate, crude fibre, ash and moisture content were determined by standard methods. Pleurotus pulminarius appeared on saw dust and cotton waste; paper, combination of saw dust and palm fruit waste, mixture of paper and cotton waste; and palm fruit waste after 2 days, 3 days and 5 days respectively. For the first stage of harvest, Saw dust had the highest length of stalk (cm) of 5.40 ± 0.63 and the lowest value of2.70 ± 0.14 was obtained from palm fruit waste only. Mixture of paper waste and cotton waste has the highest diameter of stalk (3.25 ± 0.61 cm) (second harvest), the lowest diameter of stalk (0.4 ± 0.1 cm) was recorded for palm fruit (third harvest). Cotton waste had the highest diameter of pileus (7.88 ± 1.29 cm) (first harvest) while the lowest value (1.78 ± 0.63 cm) was obtained from mushroom harvested on the mixture of paper and cotton waste (second harvest). Pleurotus pulminarius harvested on mixture of paper and cotton waste has the highest percentage protein of 40.54% while the least value 28.79% protein was obtained from mushroom harvested on saw dust. Mushroom harvested on saw dust has the highest percentage fat content of 0.99% and palm fruit waste gave the lowest percentage fat of 0.74%. Palm fruit waste displayed the highest percentage ash and crude fibre contents of 4.38% and 4.81% respectively while cotton and paper wastes had the least ash and crude fibre content of 1.81% and 0.47% respectively. Saw dust gave the highest percentage carbohydrate of 62.54%. Moisture content of 0.51% was obtained for mushrooms harvested on Palm fruit waste, Sawdust and mixture of sawdust and palm fruit waste as the least value while the mixture of paper and cotton waste has the highest moisture content of 3.49%. The study concluded that mushroom can serve as cheap source of food supplements such as protein, crude fibre, ash (micro and macro nutrients), fat, carbohydrate and moisture with beneficial bioconversion of agricultural and other wastes materials in the environment
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    Open Access
    Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife
    (International Journal of Biological Research, 2014-10) Omoboye, Olumide; Kolawole, Bakare Mufutau; Olusanjo, Adewale Isaac; Oyedeji, Olaoluwa
    Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16S rRNA gene sequencing. The bacterium with the highest α-amylase activity was selected for enzyme pro-duction. The optimal conditions for α-amylase secretion were determined by varying the pH, temperature, percentage soluble starch, nitrogen sources and carbon sources. The isolated and identified bacteria were Bacillus alvei (40%) Bacillus licheniformis (40%) and Bacillus brevis (20%) while Bacillus licheniformis RD24 was identified by 16S rRNA gene sequencing. The peak of amylase production was at 20 h of incubation (925 µg/ml/min). The optimum pH and temperature for the enzyme production were 7 and 45oC respectively. Enzyme production medium with 1% starch gave highest enzyme activity of 102 ± 5.3 µg/ml/min. Peptone gave an enzyme activity of 165 ± 8.97 µg/ml/min and yeast extract gave 52.26 ± 2.86 µg/ml/min. Of the raw starches, cassava flour gave the highest specific activity of 72 ± 0.07 Units/mg proteins, while sorghum starch gave the lowest specific activity of 5 ± 1.52 Units/mg proteins. The study conclud-ed that starch-rich household waste can be employed for amylase production using Bacillus licheniformis RD24.
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    Open Access
    Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria
    (International Journal of Biological Research, 2014-10) Omoboye, Olumide; Kolawole, Bakare Mufutau; Olusanjo, Adewale Isaac; Oyedeji, Olaoluwa
    Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16S rRNA gene sequencing. The bacterium with the highest α-amylase activity was selected for enzyme pro-duction. The optimal conditions for α-amylase secretion were determined by varying the pH, temperature, percentage soluble starch, nitrogen sources and carbon sources. The isolated and identified bacteria were Bacillus alvei (40%) Bacillus licheniformis (40%) and Bacillus brevis (20%) while Bacillus licheniformis RD24 was identified by 16S rRNA gene sequencing. The peak of amylase production was at 20 h of incubation (925 µg/ml/min). The optimum pH and temperature for the enzyme production were 7 and 45oC respectively. Enzyme production medium with 1% starch gave highest enzyme activity of 102 ± 5.3 µg/ml/min. Peptone gave an enzyme activity of 165 ± 8.97 µg/ml/min and yeast extract gave 52.26 ± 2.86 µg/ml/min. Of the raw starches, cassava flour gave the highest specific activity of 72 ± 0.07 Units/mg proteins, while sorghum starch gave the lowest specific activity of 5 ± 1.52 Units/mg proteins. The study conclud-ed that starch-rich household waste can be employed for amylase production using Bacillus licheniformis RD24.
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    Open Access
    Molecular identification and amylolytic potential of a thermophilic bacteria species from refuse dump in Ile-Ife, Nigeria
    (journal of molecular, 2014-10) Omoboye, Olumide; Kolawole, Bakare Mufutau; Olusanjo, Adewale Isaac; Olaoluwa, Oyedeji
    Molecular identification and amylolytic potential of a thermophilic bacterium species isolated from refuse dump was investigated. Bacte-rial isolates were identified by morphological and biochemical characterization while amylolytic bacterium of interest was identified by molecular analysis using 16S rRNA gene sequencing. The bacterium with the highest α-amylase activity was selected for enzyme pro-duction. The optimal conditions for α-amylase secretion were determined by varying the pH, temperature, percentage soluble starch, nitrogen sources and carbon sources. The isolated and identified bacteria were Bacillus alvei (40%) Bacillus licheniformis (40%) and Bacillus brevis (20%) while Bacillus licheniformis RD24 was identified by 16S rRNA gene sequencing. The peak of amylase production was at 20 h of incubation (925 μg/ml/min). The optimum pH and temperature for the enzyme production were 7 and 45oC respectively. Enzyme production medium with 1% starch gave highest enzyme activity of 102 ± 5.3 μg/ml/min. Peptone gave an enzyme activity of 165 ± 8.97 μg/ml/min and yeast extract gave 52.26 ± 2.86 μg/ml/min. Of the raw starches, cassava flour gave the highest specific activity of 72 ± 0.07 Units/mg proteins, while sorghum starch gave the lowest specific activity of 5 ± 1.52 Units/mg proteins. The study conclud-ed that starch-rich household waste can be employed for amylase production using Bacillus licheniformis RD24