Browsing by Author "Shittu, Adebayo O"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Open Access
    An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria
    (  Frontiers in Microbiology, 2015-10-20) Shittu, Adebayo O; Oyedara, Omotayo; Onko, Kenneth; Raji, Adeola; Peters, Georg; Müller, Lutz von; Schaumburg, Frieder; Herrmann, Mathias; Ruffing, Ulla
    Staphylococcus aureus is an important human pathogen causing nosocomial and community-acquired infections worldwide. In the characterization of this opportunistic pathogen, DNA microarray hybridization technique is used as an alternative to sequence based genotyping to obtain a comprehensive assessment on the virulence, resistance determinants, and population structure. The objective of this study was to characterize a defined collection of S. aureus isolates from Nigeria using the microarray technique, and to assess the extent that it correlates with sequence-based genotyping methods. The clonal diversity and genomic content of 52 methicillin-susceptible Staphylococcus aureus (MSSA)wereinvestigatedby spa typing,MLSTandDNAmicroarrayhybridization. More than half (55.8%) of these isolates were associated with clonal complexes (CCs) typically associated with methicillin-resistant S. aureus (MRSA) clones i.e., CC1, CC5, CC8,CC30,andCC45.Certaingeneslinkedwithvirulence(hlgAandclfA)andadherence (ebpS, fnbA, sspA, sspB, and sspP) were detected in all isolates. A number of genes or gene clusters were associated with distinct clonal types. The enterotoxin gene cluster (egc) was linked with CC5, CC25, CC30, CC45, and CC121, enterotoxin H gene (seh) with CC1, exfoliative toxin D gene (etd) with CC25 and CC80, and the epidermal cell differentiation inhibitor B gene (edinB) with CC25, CC80, and CC152. The excellent agreement between data from DNA microarray and MLST in the delineation of Nigerian MSSA isolates indicates that the microarray technique is a useful tool to provide information on antibiotic resistance, clonal diversity and virulence factors associated with infection and disease.
  • No Thumbnail Available
    Item
    Open Access
    Characterization of a Novel Thermostable Nuclease Homolog (Nuc(M)) in a Highly Divergent Staphylococcus aureus Clade
    (African Research Review. 1. 10.4314/afrrev.v1i1.40988, 2014-08) Schaumburg, Frieder; Pauly, Maude; Schubert, Grit; Shittu, Adebayo O
    A thermostable nuclease homologue (NucM) in an animal associated divergent clade of Staphylococcus aureus in sub-Saharan Africa has a highly divergent nucleotide sequence compared to classical nuc1 and nuc2 of S. aureus. The deduced amino acid sequences, tertiary structures nuclease activities are, however, similar.