Evaluation of the effect of non-enzymic antioxidant and trace element status in diabetes mellitus patients in Ile-Ife,Osun State,Nigeria
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Date
2015
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Biochemistry,Obafemi Awolowo University
Abstract
This study investigated levels of the trace elements; zinc, copper and magnesium and vitamins C and E in diabetic patients at the Obafemi Awolowo University Teaching Hospital Complex (OAUTHC), Ile- Ife. This was with a view to understanding the relationship between the levels of these parameters and the presence of diabetes and its complications.
One hundred and fifteen subjects, comprising sixty five diabetic patients and fifty healthy controls were involved in this study. Ethical clearance was obtained from the Ethical and Research Committee of OAUTHC, Ile-Ife. All subjects signed informed consent forms. Fasting blood samples of the patients and controls were collected aseptically and the plasma was collected after centrifugation at 4000 revolutions per minute (rpm) for 20 minutes. Fasting blood glucose was determined using Randox kit while glycosylated haemoglobin was determined using Clover A1c kit. Plasma aliquots were frozen at -80oC and used to analyse vitamin C, vitamin E, zinc, copper and magnesium. Vitamin C was determined by redox titration with 2, 6-dichlorophenolindophenol against plasma aliquots acidified by trichloracetic acid. Vitamin E was determined by a modified spectrophotometric method. Plasma levels of zinc, copper and magnesium were determined using standard procedures.
The mean values of fasting blood glucose, glycosylated hemoglobin (HbA1c), zinc, copper, magnesium, vitamin C and E for diabetic patients in this study were 9.12±3.86 mmol/L, 8.83±2.34 %, 6.61±4.11 µmol/L, 19.07±8.96 µmol/L, 0.64±0.07 mmol/L, 77.79±24.98 µmol/L and 1.47±1.10 µmol/L respectively while those for the healthy control subjects were 4.77±0.45 mmol/L, 15.01±4.26 µmol/L, 16.89±6.25 µmol/L, 0.77±0.03 mmol/L, 72.68±15.33 µmol/L and 4.79±1.38 µmol/L respectively. Fasting blood glucose level was significantly higher (p<0.05) in the diabetic group compared to the control group while levels of zinc, magnesium and vitamin E were significantly lower in the diabetic group compared with the controls. When the mean values of the biochemical parameters of diabetic patients with no complications, those with one microvascular complication, those with two microvascular complications and those with three microvascular complications were compared, significant differences were found in the levels of vitamin E and zinc. No significant differences were found in the parameters when the diabetic patients were grouped on the basis of glycemic control (good, fair and poor glycemic control).
This study concluded that the pattern of alterations in antioxidant trace elements and vitamins of diabetic patients appeared to be a consequence of diabetes itself, and were not predicted by glycemic status or the presence of microvascular complications.
Description
xvii,105p
Keywords
Non-enzymic, Diabetes mellitus, Trace element, Antioxidants, Glycemic status
Citation
Fagbohun,O.F(2015). Evaluation of the effect of non-enzymic antioxidant and trace element status in diabetes mellitus patients in Ile-Ife,Osun State,Nigeria. Obafemi Awolowo University