Antibacteria activties of methanolic extract of Erythrina Senegalensis DC Root Bark
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Date
2013
Authors
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Department of Microbiology, Obafemi Awolowo University
Abstract
This study investigated the antimicrobial activities of methanolic extract of Erythrina senegalensis root bark (ESRE). E. senegalensis root bark was subjected to methanolic extraction and it yielded 4.94% of crude extract. The antibacterial sensitivity test showed that the extract was effective against both Gram-positive and Gram-negative bacteria, indicating the broad-spectrum antibiotic activity of the extract. The extract was effective against 4 Gram-positive bacterial isolates and 3 Gram-negative bacterial isolates, a total of 7 bacterial isolates out of 12 isolates studied. The Gram-positive bacteria were Bacillus cereus, Bacillus subtilis, Clostridium sporogenes and Micrococcus luteus. The Gram-negative bacteria which were sensitive to the plant extract were Klebsiella pneumoniae, Escherichia coli and Pseudomonas fluorescence. The plant extract was not effective against Staphylococcus aureu, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella sp. and Shigella sp. at the concentration used (25 mg/ml). Also, methanol (5%) did not show growth inhibitory effect on the bacterial isolates.
The lowest Minimum Inhibitory Concentration (MIC) value (0.781 mg/ml) of the plant extract was observed in Bacillus subtilis and Micrococcus luteus while the highest MIC value (12.5 mg/ml) was observed in E. coli and Pseudomonas fluorescence. The Minimum Bactericidal Concentration (MBC) of the plant extract was higher than the MIC for each organism, the lowest value being 1.562 mg/ml (for Bacillus subtilis and Micrococcus luteus) and the highest MBC value being 25 mg/ml (for E. coli and Pseudomonas fluorescence). The highest percentage of bacteria killed was 85% and it was observed in Micrococcus luteus treated with the plant extract at a concentration of 3.124 mg/ml at 240 min. The lowest percentage of bacteria killed was 38% and was observed in E. coli treated with 12.5 mg/ml of the extract at 240 min.
Description
xiv,117
Keywords
Antibacteria Activities, Menthanolic Extract, Erythrina, Senegalensis Root bark
Citation
Ejelonu. A. (2013). Antibacteria activties of methanolic extract of Erythrina Senegalensis DC Root Bark. Obafemi Awolowo University