The Importance of Macrophages, T-Lymphocytes and Passive Transfer of Antibody in the Protection of Mice against Shigella Dysenteriae Type 1.

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dc.contributor.authorKoleosho, Tinuola
dc.contributor.otherShonukan, O.O.
dc.date.accessioned2014-08-26T12:19:49Z
dc.date.accessioned2018-10-29T11:18:33Z
dc.date.available2014-08-26T12:19:49Z
dc.date.available2018-10-29T11:18:33Z
dc.date.issued1987
dc.degree.awardM.Sc. Microbiologyen_US
dc.departmentMicrobiologyen_US
dc.description.abstractThe contribution of T-lymphocytes, macrophages and antibodies to protection against Shigella dysenteriae type 1 during the course of infection in mice was analysed following intraperitoneal injection of the organism into mice. Approximately 1.55 x 105 cells of Shigella dysenteriae was injected into each mouse. At intervals, the liver and spleen were dissected out to obtain fresh isolates of the organism from these organs. Ten minutes after the inoculation, ~1.59x,04 and 4.79x103 Shigella cells were recovered from the liver and spleen respectively. The number of trapped bacteria decreased from 10 mine to reach minimal levels by 6 hours and then increased to reach maximum levels by 24 hours; gradual elimination occurred after 3 days. Carrageenan (a toxic macrophage blocker) was injected into another set of mice 24hours prior to infection with Shigella. Carrageenan treatment significantly (p<0.05) enhanced the bacterial growth in the liver and spleen. This observation suggests that carrageenan sensitive macrophages are very important in the protection against Shigellosis. The role of antibody was investigated in a serum transfer experiment, AntiShigellae antibody that was raised in Rabbit was used to passively immunize another set of mice 24hours prior to the challenge with Shigellae. It was observed that although antibody was produced (titer 1:320), it had no protective role on passive transfer. The contribution of T-cells to protection was examined in another set of mice by immunosuppressing T-cells with antithymocytic serum (ATS) 24hours prior to challenge with Shigellae. The ATS was raised in Rabbit injected with thymocytes pooled from another set of mice. There was a significant (p<0.05) increase in bacterial count in ATS treated mice, showing that T-cells also play a significant role in protection against Shigella. It seems that that the protection of mice against Shigella dysenteriae depends on the synergistic action of macrophages and T-Iytphocytes.en_US
dc.facultiesScienceen_US
dc.format.filetypepdfen_US
dc.identifier.citationAPAen_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/3519
dc.language.isoenen_US
dc.pages.totalpages102pen_US
dc.publisherObafemi Awolowo Universityen_US
dc.subjectAntibodiesen_US
dc.subjectT-lymphocytesen_US
dc.subjectmacrophagesen_US
dc.subjectmiceen_US
dc.subjectorganismsen_US
dc.subjectintraperitoneal injectionsen_US
dc.subjectinoculationen_US
dc.subjectshigella dysenteriaeen_US
dc.subjectbacterial growthen_US
dc.titleThe Importance of Macrophages, T-Lymphocytes and Passive Transfer of Antibody in the Protection of Mice against Shigella Dysenteriae Type 1.en_US
dc.typeThesisen_US
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