Purification and characterisation of Lectin from Bowringia Miladbraedii Harms Seeds

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dc.contributor.authorAlakija, Augusus Adegbolamiyo Olufemi
dc.date.accessioned2020-02-10T08:51:08Z
dc.date.available2020-02-10T08:51:08Z
dc.date.issued1985
dc.description65pen_US
dc.description.abstractLectin was extracted from Bowringia mildbraedii Harms seeds and shown to agglutinate red blood cells nonspecifically. The chromatography using different grades of Sephadex did not give a very good separation of the lectin. Purification of the lectin by Sepharose 68 chromatography followed by metal chelate affinity chromatography was compared with purification by Sepharose 6B followed by ion-exchange chromatography using DEAE cellulose. The latter method was preferred and yielded a protein which behaved as a single protein on polyacrylamide gel electrophoresis. It was deduced that the lectin occured as a tetrameric protein with two subunits A and B having approximate molecular weight of 14,000 and 16,000 respectively from experiments with SOS-PAGE in the presence of 2, β-mercaptoethanol. The molecular weight of the lectin is approximately 60,000. The B. mildbraedii lectin precipitated Afzelia africana polysaccharide with remarkable specificity and failed completely to form precipitin bands in agargel double diffusion plates with other polysaccharides tested even at varying concentrations. The haemagglutinating and polysaccharide precipitating activity of the lectin is appreciably inhibited by μ-methylDmannoside, D-mannose and D-glucose.en_US
dc.identifier.citationAlakija, A.A.O. (1985). Purification and characterisation of Lectin from Bowringia Miladbraedii Harms Seeds. Obafemi Awolowo University.en_US
dc.identifier.urihttps://ir.oauife.edu.ng/handle/123456789/5171
dc.language.isoenen_US
dc.publisherDepartment of Biochemistry, Obafemi Awolowo University, Ile Ife, Nigeriaen_US
dc.subjectLectinen_US
dc.subjectBlood cellsen_US
dc.subjectAgglutinationen_US
dc.subjectChromatographyen_US
dc.subjectIon exchangeen_US
dc.subjectCelluloseen_US
dc.subjectProteinen_US
dc.subjectElectrophoresisen_US
dc.subjectHaemagglutinatingen_US
dc.subjectDiffusion platesen_US
dc.titlePurification and characterisation of Lectin from Bowringia Miladbraedii Harms Seedsen_US
dc.typeThesisen_US
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