Phenotypic and molecular characterization of multiple antibiotic-resistant escherichia coli isolated from urine samples of children patients attending mother and child hospital, Ondo, Nigeria.
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Date
2016
Authors
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Publisher
Department of Microbiology, Obafemi Awolowo University
Abstract
The study reported isolation and molecular characterization of multiple antibiotic-resistant Escherichia coli from urine samples of children patients attending Mother and Child Hospital, Ondo, Nigeria, with the view to determining the antibiogram profiling and resistance genes in multiple antibiotic resistant bacterial isolates.
A total of 300 urine samples were collected in sterile universal bottles from children patients ages between 3-5 years, which comprised 158 females and 142 males. The samples were transported on ice pack to the laboratory and analyzed bacteriologically. Isolation of E. coli and other Gram negative bacteria was carried out by streaking on Eosin Methylene Blue and MacConkey agar plates respectively, incubated at 37ºC for 24 h. Preliminary identification of isolates was by colony and morphological characteristics. Further identification of the isolates was by biochemical tests using Bergey’s manual of determinative bacteriology and Analytical Profile Index (API) Kits. Antibiotic susceptibility of isolates was done using Kirby Bauer’s disc diffusion technique. Detection of resistance genes was carried out by Polymerase Chain Reaction (PCR) using appropriate primers.
One hundred and seventeen (117) Gram negative bacterial isolates were recovered from 300 urine samples analyzed namely 35 (29.9 %) Klebsiella sp, 40 (34.2 %) Escherichia coli, 20 (17.1 %) Enterobacter sp, 13 (11.1%) Serriatia sp and 9 (7.7%) Acinetobacter sp. Resistance to antibiotics varied greatly among the E. coli isolates with resistance to Augmentin 35 (87.5 %) being the highest, Ceftriazone 32 (80 %), followed by Gentamycin 30 (75 %), Ofloxacin 28 (70%), Cefuroxime 26 (65%), Amoxicillin 16 (40%), Cefixime 11 (27.5%), Cotrimoxazole 10 (25 %), Ciprofloxacin 9 (22.5 %), Streptomycin 9 (22.5 %), Pefloxacin 8 (20%) and Ceftazidime 4 (10%) being the least. All the E. coli isolates were found to be susceptible to Nitrofurantoin, Erythromycin and Chloramphenicol. The isolates showed multiple resistance against 3 to 4 different classes of antibiotics. There was diversity in multiple antibiotic resistance pattern observed in the isolates with a total of 12 multiple antibiotic resistance patterns recorded. Eight of the twelve selected E. coli isolates had bla-CTX-M (585 bp) gene, while five haboured aac-3-IV (286 bp) gene.
The study concluded that occurrence of E. coli infection is high in the study area with high level of multiple antibiotic resistance. The recovery of resistance (bla CTX-M 585 bp and aac-3-IV 286 bp) genes in E. coli in the study is of great economic and health consequences therefore, antibiotics should only be used when prescribed by physicians
Description
xv,108
Keywords
Urine Samples, Children Patients, Mother and Child Hospital, Antibiotics-Resistant, Phenotypic Characterization, Molecular Characterization, Urine, Escherichia coli
Citation
Chukwudum, C.V. (2016). Phenotypic and molecular characterization of multiple antibiotic-resistant escherichia coli isolated from urine samples of children patients attending mother and child hospital, Ondo, Nigeria.