Tissue Culture Derived Plantlet Variation in Caladium humboldtii Schott

Simple item page
dc.contributor.authorSakpere, A. M. A.
dc.contributor.authorAdebona, A. C.
dc.coverage.geographicalNigeriaen_US
dc.date.accessioned2014-10-13T13:05:11Z
dc.date.accessioned2018-10-29T10:55:54Z
dc.date.available2014-10-13T13:05:11Z
dc.date.available2018-10-29T10:55:54Z
dc.date.issued2007
dc.departmentBotanyen_US
dc.description.abstractCallus cultures were initiated from corm and petiole explants of C. humboldtii on Murashige and Skoog's basal medium (MS) supplemented with 2,4-Dichlorophenoxyacetic acid (2, 4-D) (0.4mg/l - 1.6mg/l) in combination with Kinetin (1mg/l) in the dark. Callus was induced on media supplemented with 0.8mg/l 2,4-Dichlorophenoxyacetic acid (2, 4-D) in combination with kinetin (1mg/l) and callus induced on this media showed the best growth. Direct regeneration potential was higher in corm than in leaf explants. Regeneration was not achieved in petiole explants. De novo plant regeneration from callus cultures was not achieved and somatic embryogenesis did not yield any plantlets. Morphological differences were observed among the regenerated plantlets of C. humboldtii on Murashige and Skoog medium (MS) supplemented with 2, 4-D (0.4mg/l) in combination with 1mg/l Kinetin. Polyacrylamide gel electrophoresis showed only 1 band each in the control and in the regenerants, however, the position of the bands were different. The result indicates that variation has occurred during in vitro culture. In conclusion, it has not been possible to generate plantlets from callus and it has also not been possible to advance the callus beyond the early stage of embryo development. The findings however include production of a new cultivar of C. humboldtii, initiation/growth of callus and direct regeneration of plantlets in the dark.en_US
dc.facultiesScienceen_US
dc.format.filetypePDFen_US
dc.identifier.citationSakpere, A. M. A. and Adebona, A. C. (2007). Tissue Culture Derived Plantlet Variation in Caladium humboldtii Schott. Journal of Science and Technology, 27(1)en_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/3293
dc.language.isoenen_US
dc.pages.totalpages8en_US
dc.subjectCaladium humboldtiien_US
dc.subjectVariationen_US
dc.subjectcallusen_US
dc.subjectRegenerationen_US
dc.subjectElectrophoresisen_US
dc.titleTissue Culture Derived Plantlet Variation in Caladium humboldtii Schotten_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Tissue Culture Derived Plantlet Variation in Caladium humboldtii Schott by Sakpere and Adebona.pdf
Size:
1.31 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Thumbnail Image
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Department of Biochemistry and Molecular Biology-Journal Articles