Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa
| dc.contributor.author | Vasaikar, Sandeep | |
| dc.contributor.author | Obi, Larry | |
| dc.contributor.author | Morobe, Isaac | |
| dc.contributor.author | Bisi-Johnson, Mary | |
| dc.date.accessioned | 2020-01-24T15:45:32Z | |
| dc.date.available | 2020-01-24T15:45:32Z | |
| dc.date.issued | 2017-01 | |
| dc.description | International Journal of Microbiology, Vol. 20, No 17, Article ID 846742, p.1-7. | en_US |
| dc.description.abstract | The increase in the incidence of extended-spectrum 𝛽-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance.Theobjective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study their epidemiology. A prospective, descriptive study of 202 nonrepetitive samples frompatients was obtained fromNelson Mandela AcademicHospital.The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of 𝑏𝑙𝑎CTX-M, 𝑏𝑙𝑎TEM, 𝑏𝑙𝑎SHV, 𝑏𝑙𝑎KPC, and 𝑏𝑙𝑎NDM genes. Overall K. pneumoniae were the majority with 169 (83.7%) species isolates, followed by K. oxytoca with 29 (14.4%), while K. ozaenae and Raoultella ornithinolytica were 2 (0.9%) each. The prevalence of ESBL production in all Klebsiella species was 117 (57.9%). ESBL-genotypic resistance is driven in Mthatha by 𝑏𝑙𝑎SHV 121 (77.1%) followed by 𝑏𝑙𝑎TEM 105 (66.9%) and 𝑏𝑙𝑎CTX-M at 89 (56.7%).The most common ESBL genotype combination among the Klebsiella was 𝑏𝑙𝑎TEM + 𝑏𝑙𝑎SHV + 𝑏𝑙𝑎CTX-M at 79 (50.3%).There is a steady increase in the rate of ESBL genes in the last five years. | en_US |
| dc.identifier.other | DOI: 10.1155 | |
| dc.identifier.uri | https://ir.oauife.edu.ng/handle/123456789/5131 | |
| dc.language.iso | en | en_US |
| dc.publisher | International Journal of Microbiology | en_US |
| dc.subject | Klebsiella | en_US |
| dc.subject | Antibiotic Resistance | en_US |
| dc.subject | South Africa | en_US |
| dc.subject | Cape Province | en_US |
| dc.title | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa | en_US |
| dc.type | Journal | en_US |