An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria
| dc.contributor.author | Shittu, Adebayo O | |
| dc.contributor.author | Oyedara, Omotayo | |
| dc.contributor.author | Onko, Kenneth | |
| dc.contributor.author | Raji, Adeola | |
| dc.contributor.author | Peters, Georg | |
| dc.contributor.author | Müller, Lutz von | |
| dc.contributor.author | Schaumburg, Frieder | |
| dc.contributor.author | Herrmann, Mathias | |
| dc.contributor.author | Ruffing, Ulla | |
| dc.date.accessioned | 2023-05-13T17:53:43Z | |
| dc.date.available | 2023-05-13T17:53:43Z | |
| dc.date.issued | 2015-10-20 | |
| dc.description | Frontiers in Microbiology,Vol. 6,Article 1160,p.10 | en_US |
| dc.description.abstract | Staphylococcus aureus is an important human pathogen causing nosocomial and community-acquired infections worldwide. In the characterization of this opportunistic pathogen, DNA microarray hybridization technique is used as an alternative to sequence based genotyping to obtain a comprehensive assessment on the virulence, resistance determinants, and population structure. The objective of this study was to characterize a defined collection of S. aureus isolates from Nigeria using the microarray technique, and to assess the extent that it correlates with sequence-based genotyping methods. The clonal diversity and genomic content of 52 methicillin-susceptible Staphylococcus aureus (MSSA)wereinvestigatedby spa typing,MLSTandDNAmicroarrayhybridization. More than half (55.8%) of these isolates were associated with clonal complexes (CCs) typically associated with methicillin-resistant S. aureus (MRSA) clones i.e., CC1, CC5, CC8,CC30,andCC45.Certaingeneslinkedwithvirulence(hlgAandclfA)andadherence (ebpS, fnbA, sspA, sspB, and sspP) were detected in all isolates. A number of genes or gene clusters were associated with distinct clonal types. The enterotoxin gene cluster (egc) was linked with CC5, CC25, CC30, CC45, and CC121, enterotoxin H gene (seh) with CC1, exfoliative toxin D gene (etd) with CC25 and CC80, and the epidermal cell differentiation inhibitor B gene (edinB) with CC25, CC80, and CC152. The excellent agreement between data from DNA microarray and MLST in the delineation of Nigerian MSSA isolates indicates that the microarray technique is a useful tool to provide information on antibiotic resistance, clonal diversity and virulence factors associated with infection and disease. | en_US |
| dc.identifier.other | 10.3389/fmicb.2015.01160 | |
| dc.identifier.uri | https://ir.oauife.edu.ng/123456789/5530 | |
| dc.language.iso | en | en_US |
| dc.publisher | Frontiers in Microbiology | en_US |
| dc.subject | Nigeria | en_US |
| dc.subject | genotyping | en_US |
| dc.subject | MLST | en_US |
| dc.subject | microarray | en_US |
| dc.subject | Staphylococcus aureus | en_US |
| dc.title | An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria | en_US |
| dc.type | Journal | en_US |