The anti-hyperlipidemic effect of a formulated diet containing Tetrapleura tetraptera fruits and Xanthosoma sagittifolium leaves in adult wistar rats
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Date
2016
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Biochemistry,Obafemi Awolowo University
Abstract
This study investigated the hypolipidemic effect, singly and combined, of enriched diet containing Tetrapleura tetraptera fruits extract and Xanthosoma sagittifolium leaves and also their influence on the liver function. This was with a view to exploiting these plants in the development of antihyperlipidemic agents.
Dry fruits of Tetrapleura tetraptera and Xanthosoma sagittifolium leaves were collected from Obafemi Awolowo University campus, Ile-Ife. Tetrapleura tetraptera fruits were pulverized and ethanolic extract was obtained using 70% ethanol. The extract was the freeze-dried. Xanthosoma sagittifolium leaves were washed, sun-dried and powdered. The experimental feeds were enriched with 0.5% T. tetraptera ethanolic extract (TTE) powder and 2% X. sagittifolium leaf (XSL) powder. Forty-two male albino rats (average weight; 144.04 g) were obtained and allotted into seven groups of six animals each and subjected to treatment regimen for a period of forty-two days as follows: Groups A, B and G were fed standard chow, Group C was fed 0.5% T. tetraptera diet, Group D, 2% X. sagittifolium, Groups E and F received diet containing 0.5% T. tetraptera and 2% X. sagittifolium. Group G received oral administration of rosuvastatin (a standard drug of choice used for the management of hyperlipidemia) daily. At the end of the treatment period, hyperlipidemia was induced in groups B, F and G by a single dose of 250mg/kg body weight of Tyloxapol administered intraperitoneally. Animals were then sacrificed. The serum and the liver were collected and assayed for total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C). Differences between mean values were analysed using the Student t-Test and differences were considered to be significance when p < 0.05.
The results showed that the combination of T. tetraptera and X. sagittifolium in diet produced an additive effect in the reduction of hepatic cholesterol and triglyceride levels in Group E. Also, the significant (p < 0.05) increase in serum and hepatic TC, TG and LDL caused by the administration of tyloxapol was significantly nullified by the combined TTE and XSL diet. The combination of T. tetraptera and X. sagittifolium elicited a significant (p < 0.05) percentage reduction in serum TC, TG and LDL by 44.28%, 40.25% and 43.34% respectively and increased the level of HDL by 34.47% in Group F compared to Group B. The enriched diet also showed reduction in the TC/HDL ratio (1.453 ± 0.26) in Group F compared to hyperlipidemic rats.
The study concluded that the combined use of dietary T. tetraptera extract and X. sagittifolium leaves in normal and hyperlipidemic rats showed anti-hyperlipidemic effect.
Description
xvi,108p
Keywords
Hyperlipidemic rats, Anti-hyperlipidemic, Tetrapleura tetraptera, Xanthosoma sagittifolium, Wistar rats
Citation
Odejide,I.T (2016). The anti-hyperlipidemic effect of a formulated diet containing Tetrapleura tetraptera fruits and Xanthosoma sagittifolium leaves in adult wistar rats.Obafemi Awolowo University