Studies on protease from Aspergillus Aculeatus

Simple item page
dc.contributor.authorElegbe, Isaac Anuolowapo
dc.date.accessioned2023-05-13T16:38:03Z
dc.date.available2023-05-13T16:38:03Z
dc.date.issued1985
dc.description183pen_US
dc.description.abstractAspergillus aculeatus associated with the black tongue disease was able to grow in liquid synthetic medium. Growth was maximum on the fifth day of incubation. Growth was optimum at pH 6.5 and 35 °c. The best carbon source for growth was glucose while among the nitrogen sources used, optimum growth occurred on tryptone. During growth of A. aculeatus in liquid synthetic medium, proteins were released and proteolytic activity was detected. Proteolytic activity was optimum when the sole carbon and nitrogen sources were galactose (or inositol) and peptone respectively. The proteins were separated into three peaks of absorption by gel permeation chromatography, and only one of the components exhibited proteolytic activity. The molecular weight of this protease from its elution volume on Sephadex G-100 was approximately 28,500 Daltons. Ion exchange chromatography resolved only one peak of absorption with a purification fold of approximately 309.5, a specific activity of 2.7 x 10' units/mg protein, and a Km of approximately 0.26 mg/ml for the hydrolysis of casein. The partially purified enzyme was able to hydrolyse casein, lactalbumin, egg albumin and human haemoglobin. Optimum activity of the enzyme occurred at pH 6. 5 and 40 °C. Activity of the enzyme was stimulated by low concentrations of Cu++, Ba++, Ca++, Mg++ and K+ but inhibited by Mn++, Zn +, Fe +, Hg++, iodoacetic acid and ethylene diamine tetraacetic acid. The enzyme was highly susceptible to heat, loosing all its activity within 2 minutes at 70 °C. Intraperitoneal inoculation of white rats (Fisher's breed) with the crude and the partially purified enzyme caused marked histological changes resulting in cellular degeneration and local necrosis of three major organs (liver, kidney and spleen). The partially purified enzyme was more effective than the crude enzyme, and the changes produced by both enzymes were more pronounced in the male than in the female ratsen_US
dc.identifier.urihttps://ir.oauife.edu.ng/123456789/5300
dc.language.isoenen_US
dc.publisherObafemi Awolowo University, Ile Ife, Nigeriaen_US
dc.subjectDiseaseen_US
dc.subjectIncubationen_US
dc.subjectCarbonen_US
dc.subjectGrowthen_US
dc.subjectGalactoseen_US
dc.subjectProteinsen_US
dc.subjectChromatographyen_US
dc.subjectIonen_US
dc.subjectEnzymesen_US
dc.subjectHeaten_US
dc.subjectCellular degenerationen_US
dc.subjectIntraperitoneal inoculationen_US
dc.titleStudies on protease from Aspergillus Aculeatusen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Studies on protease from Aspergillus Aculeatus- Ph.pdf
Size:
44.93 KB
Format:
Adobe Portable Document Format
Description:
P.h.D. Microbiology
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Ph.D