An Evaluation of Damage to Bovine Spermatozoa during Processing, Freezing and Thawing. I Leakage of Glutamic-oxaloactetic transaminase [GOT] from Spermatozoa
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Date
1979
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Faculty of Agriculture, University of Ife
Abstract
The activity of glutamic-oxaloacetic transaminase (GOT) in the seminal plasma of thirty ejaculates after collection; after extension, cooling and equilibration just before freezing; and after freezing and thawing were determined. After correction for the reduction in the activity of GOT caused by the extender and low temperatures the activity of the enzyme was higher (P 0.05) in post thaw semen than in either ejaculated or equilibrated semen just before freezing. However, the pre-freeze leakage of GOT from spermatozoa was similar to the leakage caused by freezing and thawing. The significance of these findings in relation to the use, of GOT activity in seminal plasma as an index of spermatozoa damage were discussed.
Description
Keywords
Bull semen, Seminal plasma, Spermatozoa, Bovine semen
Citation
Somade, B.; King, G. J. and Machperson, J. W. (1979). An Evaluation of Damage to Bovine Spermatozoa during Processing, Freezing and Thawing. I Leakage of Glutamic-oxaloactetic transaminase [GOT] from Spermatozoa. Ife Journal of Agriculture, 1(1).