Post-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Pat

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dc.contributor.authorFagbohun, Dayo Emman
dc.contributor.otherOgundana, S. K.
dc.date.accessioned2014-06-25T09:45:14Z
dc.date.accessioned2018-10-29T11:18:09Z
dc.date.available2014-06-25T09:45:14Z
dc.date.available2018-10-29T11:18:09Z
dc.date.issued1986
dc.degree.awardM.Sc. Microbiologyen_US
dc.departmentMicrobiologyen_US
dc.description.abstractFour phytoalexins were induced D. rotundata in post-infectionally with B. theobromae. Three of the Phytoalexins were isolated and identified with the aid of ultra-violet mass and nuclear magnetic resonance spectral data as 3,2,5 trihydroxydiphenyl 1 , 2 ethane; 3, 5 dihydroxydiphenyl 1,2 ethane; 1,5 dihydroxy-3-methoxydiphenyl 1,2 ethane and an uncharacterised. Maximum production of the phytoalexins was achieved at 30 hours of incubation. Chloroform: methanol, (96:4 v/v) gave the best separation of the phytoalexins from yam crude extract. Antifungal activities of the phytoalexins against spore germination varied according to fungal species but the pores were more sensitive to 3, 2, 5 trihydroxydiphenyl 1,2 ethane than 3, 5 dihydroxydiphenyl 1,2 ethane and the spores of B. theobromae were least affected. The ED50 values required to prevent spore germination of the pathogen varied from 45.7 µg/ml 58.9 µg/ml for 3,2, 5 trihydroxydiphenyl 1,2 ethane and 45.6 µg/ml to 81.3 µg/ml ;3,5 dihydroxydiphenyl 1,2 ethane. The ED50 value of the two phytoalexins to prevent germ tube elongation of the yam rot pathogens was highest for B. theobromae (61.07 µg/ml) and least for A.niger (46.77 µg/ml). The compound 3,2, 5 trihydroxydiphenyl 1 ,2 ethane was found to be active against the four bacterial species tested against it even at the low concentration of 10 µg/ml.en_US
dc.facultiesScienceen_US
dc.format.filetypepdfen_US
dc.identifier.citationAPAen_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/3516
dc.language.isoenen_US
dc.pages.totalpages110pen_US
dc.publisherObafemi Awolowo Universityen_US
dc.subjectInfectionen_US
dc.subjectphytoalexinsen_US
dc.subjectultra-violet massen_US
dc.subjectnuclear magnetic resonance spectral dataen_US
dc.subjectincubationen_US
dc.subjectchloroformen_US
dc.subjectmethanolen_US
dc.subjectyam crude extracten_US
dc.titlePost-infectional Production of Phytoalexin in White Yam (Discorea Rotundata Linn.) Innoculated with Botryodiplodia Theobromae Paten_US
dc.typeThesisen_US
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