Purification and Characterization of Lectins from Abrus Species.

Simple item page
dc.contributor.authorCaiquo, Aspect Wallace Kyemenu
dc.contributor.otherAnimashaun, T.
dc.date.accessioned2014-06-25T10:57:52Z
dc.date.accessioned2018-10-29T10:56:06Z
dc.date.available2014-06-25T10:57:52Z
dc.date.available2018-10-29T10:56:06Z
dc.date.issued1985
dc.departmentBiochemistryen_US
dc.description.abstractSaline extracts prepared from Abrus precatorius and Abrus fructiculosus seeds agglutinated red blood cells. The agglutination was inhibited by D-galactose and lactose. The lectins bound to Sepharose 6B and were eluted with D-galactose. These lectins were separated into an agglutinin and a toxin by chromatography on Diethyl amino ethyl (DEAE) cellulose. The toxin from Abrus fructiculosus seed was named fructin' to distinguish it from Abrus precatorius seed toxin, abrin. In sodium dodecyl - polyacrylamide gel electrophoresis, (SDS-PAGE) fructin and abrin gave single bands with molecular weights (proposed) 60,320 and 62,500 respectively. After treatment with mercaptoethanol, fructin and abrin were split into 2 bands each with molecular weights 32,360 and 30,200 (fructin), 36,520 and 28,800 (abrin). The agglutinins gave 2 bands each in SDS-PAGE with corresponding molecular weights of 57,480 and 55,650 (A. fructiculosus agglutinin) and 59,600 and 56,380 (A. precatorius agglutinin). The proposed native molecular weights are 113,130 (. fructiculosus agglutinin) and 115,980 ( A. precatorius agglutinin), Alen treated with SDS and f - mercaptoethanol, the agglutinins were split into 3 bands each with corresponding agar molecular weights 38,460, 33,500 and 28,180 (,. fructiculosus agglutinin) and 40,790, 35,590 and 31,260 (A.precatorius agglutinin). The saline extracts from the two seeds did not interact with Afzelia africana polysaccharide in agar gel double diffusion studies. The toxicity of fructin and A. fructiculosus agglutinin was established on mice, with fructin being about 150 times more toxic than the agglutinin. Antisera formed against abrin and A. precatorius agglutinin did not interact with saline extracts of A. fructiculosus, fructin and A. fructiculosus agglutinin.en_US
dc.facultiesScienceen_US
dc.format.filetypepdfen_US
dc.identifier.citationAPAen_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/3298
dc.language.isoenen_US
dc.matric.numberM.Sc. Biochemistryen_US
dc.pages.totalpages76pen_US
dc.publisherObafemi Awolowo Universityen_US
dc.subjectRed blood cellsen_US
dc.subjectagglutinationen_US
dc.subjectchromatographyen_US
dc.subjectdiffusionen_US
dc.subjectlectinsen_US
dc.subjectsalineen_US
dc.subjectfructinen_US
dc.subjectabrinen_US
dc.titlePurification and Characterization of Lectins from Abrus Species.en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Purification and characterization of lectins from Abrus species-M.Sc.pdf
Size:
38.02 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Thumbnail Image
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses and Dissertations