In vitro antioxidant activity and gas chromatographymass spectrometry analysis of solvent extracts of Kigelia africana stem bark
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Date
2017-07-20
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Abstract
Objective: Kigelia africana is widely used, traditionally, in Africa in the treatment of many conditions and complaints such
as kidney and stomach disorders, malaria, wounds, and venereal diseases. In the present study, an attempt has been made to
investigate the antioxidant activity of extracted fractions of the stem bark and analyze the non-polar fractions for the presence
of various components that may be responsible for their antioxidant properties. Methods: The antioxidant activity was
evaluated using 1,1-diphenyl-2-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation and
nitric oxide radical scavenging models. Analysis of the chemical components of the solvent extracts was carried out by gas
chromatography-mass spectrometry (GC-MS). Results: Strong activity (71-89% inhibition) was found for the ethyl acetate
fraction, whereas the non-polar chloroform fraction exhibited a relatively weak activity (24-48%) inhibition, at 25 μg/ml
concentration. GC-MS analysis of the non-polar extracted fractions identified 21 and 12 compounds for the hexane and
chloroform fractions, respectively, of which α-terpineol (2) (39.21%) was the main component identified for the hexane
extract, and α-amyrin (9) (32.54%) was the main component identified for the chloroform extract. Conclusion: This study
shows that the non-polar extracted fractions of K. africana stem bark may be a potential source of natural antioxidants or
bioactive agents.
Description
11p
Keywords
Antioxidant activity, Gas chromatography-mass spectrometry, Kigelia africana, Non-polar solvent extract, Percentage inhibition
Citation
Obafemi. C.A. et al. (2017). In vitro antioxidant activity and gas chromatographymass spectrometry analysis of solvent extracts of Kigelia africana stem bark. Journal of Pharmacy Research 11(8):973-982