Browsing by Author "Akindahunsi, Afolabi Akintunde"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Open Access
    Synthesis and characterization of poly-5- dimethylaminouridylic acid
    (Obafemi Awolowo University, Ile Ife, Nigeria, 1985) Akindahunsi, Afolabi Akintunde
    The bromination of uridine monophosphate (UMP) with Br2 in dry formamide gave 5- bromouridine monophosphate (Br5UMP), which by reaction with dimethylamine was converted into 5-dimethylaminouridine monophosphate (Me2N5UMP). This was treated with phosphoric acid by the phosphoromorpholidate method to afford 5-dimethylaminouridine -5'-diphosphate (Me2N5UDP). Poly 5-dimethylaminouridylic acid, otherwise known as Poly (Me2N5U) was prepared by polymerization of 5-dimethylaminouridine diphosphate (Me2N5UDP) with polynucleotide phosphorylase (PNPase). The polymer formed a 1:1 hybrid with polyadenylic acid (Poly A).