Synthesis and characterization of poly-5- dimethylaminouridylic acid
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Date
1985
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Obafemi Awolowo University, Ile Ife, Nigeria
Abstract
The bromination of uridine monophosphate (UMP) with Br2 in dry formamide gave 5-
bromouridine monophosphate (Br5UMP), which by reaction with dimethylamine was converted
into 5-dimethylaminouridine monophosphate (Me2N5UMP). This was treated with phosphoric
acid by the phosphoromorpholidate method to afford 5-dimethylaminouridine -5'-diphosphate
(Me2N5UDP).
Poly 5-dimethylaminouridylic acid, otherwise known as Poly (Me2N5U) was prepared by
polymerization of 5-dimethylaminouridine diphosphate (Me2N5UDP) with polynucleotide
phosphorylase (PNPase). The polymer formed a 1:1 hybrid with polyadenylic acid (Poly A).
Description
66p
Keywords
Bromination, Polymerization, Uridine monophosphate, Acid