Browsing by Author "King, G. J."

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    An Evaluation of Damage to Bovine Spermatozoa during Processing, Freezing and Thawing II. Effect on Motility, Percentage Live and Ultrastructure of Spermatozoa
    (Faculty of Agriculture, University of Ife, 1979) Somade, B.; King, G. J.; Macpherson, J. W.
    Thirty semen samples from five bulls were processed and frozen. Each ejaculate was evaluated for percent progressive motility, percent live spermatozoa and percentage of heads, tails and mid-pieces with intact plasma membrane after collections, after extension and cooling just before freezing and after thawing. The greatest decrease in both motility and percent live spermatozoa occurred before freezing. More spermatozoa heads were damaged at each phase of processing for freezing than mid-pieces and tails and there was also a greater number of damaged spermatozoa before freezing than after freezing and thawing.
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    An Evaluation of Damage to Bovine Spermatozoa during Processing, Freezing and Thawing. I Leakage of Glutamic-oxaloactetic transaminase [GOT] from Spermatozoa
    (Faculty of Agriculture, University of Ife, 1979) Somade, B.; King, G. J.; Machperson, J. W.
    The activity of glutamic-oxaloacetic transaminase (GOT) in the seminal plasma of thirty ejaculates after collection; after extension, cooling and equilibration just before freezing; and after freezing and thawing were determined. After correction for the reduction in the activity of GOT caused by the extender and low temperatures the activity of the enzyme was higher (P 0.05) in post thaw semen than in either ejaculated or equilibrated semen just before freezing. However, the pre-freeze leakage of GOT from spermatozoa was similar to the leakage caused by freezing and thawing. The significance of these findings in relation to the use, of GOT activity in seminal plasma as an index of spermatozoa damage were discussed.