Department of Microbiology-Journal Articles

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Browsing Department of Microbiology-Journal Articles by Subject "Cape Province"

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    Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa
    (International Journal of Microbiology, 2017-01) Vasaikar, Sandeep; Obi, Larry; Morobe, Isaac; Bisi-Johnson, Mary
    The increase in the incidence of extended-spectrum 𝛽-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance.Theobjective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study their epidemiology. A prospective, descriptive study of 202 nonrepetitive samples frompatients was obtained fromNelson Mandela AcademicHospital.The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of 𝑏𝑙𝑎CTX-M, 𝑏𝑙𝑎TEM, 𝑏𝑙𝑎SHV, 𝑏𝑙𝑎KPC, and 𝑏𝑙𝑎NDM genes. Overall K. pneumoniae were the majority with 169 (83.7%) species isolates, followed by K. oxytoca with 29 (14.4%), while K. ozaenae and Raoultella ornithinolytica were 2 (0.9%) each. The prevalence of ESBL production in all Klebsiella species was 117 (57.9%). ESBL-genotypic resistance is driven in Mthatha by 𝑏𝑙𝑎SHV 121 (77.1%) followed by 𝑏𝑙𝑎TEM 105 (66.9%) and 𝑏𝑙𝑎CTX-M at 89 (56.7%).The most common ESBL genotype combination among the Klebsiella was 𝑏𝑙𝑎TEM + 𝑏𝑙𝑎SHV + 𝑏𝑙𝑎CTX-M at 79 (50.3%).There is a steady increase in the rate of ESBL genes in the last five years.