ATR-FTIR AND HPLC SPECTROSCOPIC STUDIES AND EVALUATION OF MINERAL CONTENT OF CARICA PAPAYA LEAVES AND FLOWERS
We applied Fourier transform infrared spectroscopy (FTIR) to study the main constituents in the male Carica papaya leaves and flowers in the mid infrared region 4000–650 cm –1. The findings indicated that FTIR spectrum can discriminate and identify various functional groups present in the pawpaw parts. Four water-soluble vitamins, thiamine (vitamin B 1 ), riboflavin (vitamin B 2 ) niacin (vitamin B3) and folic acid (vitamin B9) were analyzed by HPLC. Niacin and folic acid were detected in the leaves at a concentration of 7.08 mg/100 g and 1.00 mg/100 g dry weight, respectively, while only folic acid could be detected in the flowers at a high concentration of 510.34 mg/100 g dry weight. Atomic absorption spectroscopy (AAS) analysis of the samples showed that the leaves and flowers contain elements like K, Na, Mn, Mg, Fe, Zn, Cu, Ca, Cd and Ni in various proportions, with the leaves containing higher concentrations of Mg, Ca, Zn, Fe and Ni than the flowers.