Regeneration of Adiantum Capillus-Veneris L.: an homosporous fern species.

Olatunji, Tosin Yetunde (2014)

xi,59

Thesis

Spore and tissue culturestudies ofAdiantum capillus-veneris, an ornamental and medicinal fern species were carried outto raise new fernlets, study the growth rate and develop a protocol for rapid vegetative propagation of the fern in-vitro. Sterilized spores were sown on semi-solid agar containing Parker’s and Thompson’s nutrient. Agar and soil cultures were established to study spore germination and gametophyte development. The germination rate was scored at an interval of four days for thirty days.Sporophyte and gametophyte explants were sterilized and cultured onMurashige and Skoog (MS) medium supplemented with different auxins (IBA and NAA) and cytokinins (BA and Kinetin). Callus regeneration was obtained from leaflet explants cultured on MS medium supplemented with 0.50 mg/L NAA. Root regeneration was obtained from rhizome explants cultured on MS medium supplemented with 0.5 mg/L IBA and MS medium supplemented with 0.5 mg/L NAA. Shoot regeneration was obtained from stipe explant cultured on MS medium supplemented with 0.15 mg/L KIN. Crozier explants gave rise to shoots in MS medium supplemented with 0.5 mg/L BA. Direct regeneration of plantlets was obtained from rhizome explants supplemented with 0.25 mg/L BA and crozier explant cultured on MS medium supplemented with 0.5 mg/L IBA. Neither callogenesis nor organogenesis was observed from gametophyte explants. This study showed that A. capillus-veneris can be raised from spores. A protocol for efficient regeneration of A. capillus-veneris has been developed using rhizome explant in BA and crozier segment in IBA.

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