Phofluorescence lifetime measurement by single photon counting
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Date
1985
Authors
Journal Title
Journal ISSN
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Publisher
Obafemi Awolowo University, Ile Ife, Nigeria
Abstract
A fluorescence lifetime measuring instrument in the nanosecond time range has been assembled
using components from the ORTEC, EMI, TEKTRONIX, BRANDENBURG AND
CAMBERRA. The fluorescence emmission spectra of two aromatic hydro-carbons (naphthalene
and pyrene) wore measured for identification and proper classification.
The lifetime measuring instrument was calibrated and tested with 1μg/ml solution of quinine
sulphate in. 0.05M sulphuric acid. A value of 21.50 ns was obtained for the fluorescence lifetime
of quinine sulphate. The fluorescence lifetimes of naphthalene and pyrene were then determined
with this system using four different concentration of each sample. A vacuum system was also
constructed for removing the dissolved oxygen in these aromatic hydrocarbons.
After degassing the measurement was repeated for each sample. The monomer fluorescence
lifetime of 437.50 ns was obtained for naphthalene while 577.02 ns was obtained for pyrene.
Description
134pg
Keywords
Hydrocarbon, Naphthalene, Oxygen, Monomer fluorescence, Nanosecond, Calibration, Pyrene